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Title: | Regeneration of Melia volkensii Gurke (Meliaceae) through direct somatic embryogenesis |
Authors: | Indieka, Abwao S. Odee, David W. Muluvi, G.M. |
Keywords: | Cotyledon mass-propagation seed storage somatic embryos zygotic embryos |
Issue Date: | 2007 |
Publisher: | Springer |
Series/Report no.: | New forests;Vol.34: 73-81 |
Abstract: | Experiments were conducted to study plant regeneration through direct somatic embryogenesis using mature zygotic embryo and cotyledonary explants from seeds of Melia volkensii stored for <3 and >12 months. Explants were cultured on Murashige and Skoog (MS) medium supplemented with BAP, NAA and 2,4-0 (0.5,1.0 and 2.0 mg rl) alone, and BAP (0.5, 1.0, 2.0 and 4.0 mg I-I) in combination with 2,4-0 or NAA (0.2 and 0.5 mg 1-1). After 4 weeks in culture, up to 60% of cotyledonary explants from the seeds stored for <3 months produced direct somatic embryos on BAP (0.5-4.0 mg I-I) in combination with 2,4-0 (0.2 mg rl). The number of somatic embryos ranged from 5 to 14 per explant in BAP (0.5 mg rl ) and 2,4-0 (0.2 mg 1-1) combination. Only 20% of cotyledonary explants from seeds stored for >12 months produced somatic embryos. Mature zygotic embryos failed to produce any somatic embryos. Subcultures of somatic embryos from cotyledonary explants of seeds stored for <3 months formed clusters of shootlets on semi solid MS and 1/2 MS media. After 6 weeks of subculture on multiplication MS media augmented with BAP (0.5 mg I-I) and IAA (0.2 mg rl ), 70% of the shoot tips formed 4-7 shoots per explant. Up to 33% of the multiplied shoots were rooted in MS |
URI: | http://10.10.20.22:8080//handle/123456789/559 |
Appears in Collections: | Journals |
Files in This Item:
File | Description | Size | Format | |
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REGENERATION OF MELIA VOLKENSII GURKE ABSTRACT.pdf | 463.2 kB | Adobe PDF | View/Open |
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