Please use this identifier to cite or link to this item: http://localhost:80/jspui/handle/123456789/559
Title: Regeneration of Melia volkensii Gurke (Meliaceae) through direct somatic embryogenesis
Authors: Indieka, Abwao S.
Odee, David W.
Muluvi, G.M.
Keywords: Cotyledon
mass-propagation
seed storage
somatic embryos
zygotic embryos
Issue Date: 2007
Publisher: Springer
Series/Report no.: New forests;Vol.34: 73-81
Abstract: Experiments were conducted to study plant regeneration through direct somatic embryogenesis using mature zygotic embryo and cotyledonary explants from seeds of Melia volkensii stored for <3 and >12 months. Explants were cultured on Murashige and Skoog (MS) medium supplemented with BAP, NAA and 2,4-0 (0.5,1.0 and 2.0 mg rl) alone, and BAP (0.5, 1.0, 2.0 and 4.0 mg I-I) in combination with 2,4-0 or NAA (0.2 and 0.5 mg 1-1). After 4 weeks in culture, up to 60% of cotyledonary explants from the seeds stored for <3 months produced direct somatic embryos on BAP (0.5-4.0 mg I-I) in combination with 2,4-0 (0.2 mg rl). The number of somatic embryos ranged from 5 to 14 per explant in BAP (0.5 mg rl ) and 2,4-0 (0.2 mg 1-1) combination. Only 20% of cotyledonary explants from seeds stored for >12 months produced somatic embryos. Mature zygotic embryos failed to produce any somatic embryos. Subcultures of somatic embryos from cotyledonary explants of seeds stored for <3 months formed clusters of shootlets on semi solid MS and 1/2 MS media. After 6 weeks of subculture on multiplication MS media augmented with BAP (0.5 mg I-I) and IAA (0.2 mg rl ), 70% of the shoot tips formed 4-7 shoots per explant. Up to 33% of the multiplied shoots were rooted in MS
URI: http://10.10.20.22:8080//handle/123456789/559
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